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faps  (R&D Systems)


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    Structured Review

    R&D Systems faps
    Faps, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fap+antibody/Human+PDGF+R+alpha+Antibody/pmc13082186-78-68-75
    Average 93 stars, based on 79 article reviews
    faps - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: The fibrotic area and CSA were quantified using ImageJ 1.50i software (National Institutes of Health, Bethesda, MD, USA). .. Immunohistochemical Analysis To evaluate the expression of FAP, human and mouse cardiac tissues were deparaffinized, blocked with 5% BSA, and incubated with an anti-FAP antibody (AF3715, R&D Systems) overnight at 4 °C. .. Subsequently, all the specimens were incubated with fluorescent secondary antibodies (ab150177, Abcam, Cambridge, UK) for 1 h at room temperature.

    Expressing:

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: The fibrotic area and CSA were quantified using ImageJ 1.50i software (National Institutes of Health, Bethesda, MD, USA). .. Immunohistochemical Analysis To evaluate the expression of FAP, human and mouse cardiac tissues were deparaffinized, blocked with 5% BSA, and incubated with an anti-FAP antibody (AF3715, R&D Systems) overnight at 4 °C. .. Subsequently, all the specimens were incubated with fluorescent secondary antibodies (ab150177, Abcam, Cambridge, UK) for 1 h at room temperature.

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: The fibrotic area and CSA were quantified using ImageJ 1.50i software (National Institutes of Health, Bethesda, MD, USA). .. To evaluate the expression of FAP, human and mouse cardiac tissues were deparaffinized, blocked with 5% BSA, and incubated with an anti-FAP antibody (AF3715, R&D Systems) overnight at 4 °C. .. Subsequently, all the specimens were incubated with fluorescent secondary antibodies (ab150177, Abcam, Cambridge, UK) for 1 h at room temperature.

    Incubation:

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: The fibrotic area and CSA were quantified using ImageJ 1.50i software (National Institutes of Health, Bethesda, MD, USA). .. Immunohistochemical Analysis To evaluate the expression of FAP, human and mouse cardiac tissues were deparaffinized, blocked with 5% BSA, and incubated with an anti-FAP antibody (AF3715, R&D Systems) overnight at 4 °C. .. Subsequently, all the specimens were incubated with fluorescent secondary antibodies (ab150177, Abcam, Cambridge, UK) for 1 h at room temperature.

    Article Title: Enhancement of drug delivery through fibroblast activation protein-targeted near-infrared photoimmunotherapy.
    Article Snippet: .. For FAP and sibrotuzumab, the cells were incubated with PE-conjugated anti-FAP antibody (FAB3715P, R&D Systems) (1:50), PE-conjugated isotype control (IC002P, R&D Systems) (1:50), or Sib-IR700 conjugate (10 μg/mL) for 30 minutes. .. After staining, the cells were fixed with 4% paraformaldehyde (PFA) for 30 minutes and analyzed using a FACSLyric flow cytometer (BD Biosciences Inc.), with the PE and APC-Cy7 channels.

    Article Title: Enhancement of drug delivery through fibroblast activation protein–targeted near-infrared photoimmunotherapy
    Article Snippet: .. For FAP and sibrotuzumab, the cells were incubated with PE-conjugated anti-FAP antibody (FAB3715P, R&D Systems) (1:50), PE-conjugated isotype control (IC002P, R&D Systems) (1:50), or Sib-IR700 conjugate (10 μg/mL) for 30 minutes. .. After staining, the cells were fixed with 4% paraformaldehyde (PFA) for 30 minutes and analyzed using a FACSLyric flow cytometer (BD Biosciences Inc.), with the PE and APC-Cy7 channels.

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: The fibrotic area and CSA were quantified using ImageJ 1.50i software (National Institutes of Health, Bethesda, MD, USA). .. To evaluate the expression of FAP, human and mouse cardiac tissues were deparaffinized, blocked with 5% BSA, and incubated with an anti-FAP antibody (AF3715, R&D Systems) overnight at 4 °C. .. Subsequently, all the specimens were incubated with fluorescent secondary antibodies (ab150177, Abcam, Cambridge, UK) for 1 h at room temperature.

    Control:

    Article Title: Enhancement of drug delivery through fibroblast activation protein-targeted near-infrared photoimmunotherapy.
    Article Snippet: .. For FAP and sibrotuzumab, the cells were incubated with PE-conjugated anti-FAP antibody (FAB3715P, R&D Systems) (1:50), PE-conjugated isotype control (IC002P, R&D Systems) (1:50), or Sib-IR700 conjugate (10 μg/mL) for 30 minutes. .. After staining, the cells were fixed with 4% paraformaldehyde (PFA) for 30 minutes and analyzed using a FACSLyric flow cytometer (BD Biosciences Inc.), with the PE and APC-Cy7 channels.

    Article Title: Enhancement of drug delivery through fibroblast activation protein–targeted near-infrared photoimmunotherapy
    Article Snippet: .. For FAP and sibrotuzumab, the cells were incubated with PE-conjugated anti-FAP antibody (FAB3715P, R&D Systems) (1:50), PE-conjugated isotype control (IC002P, R&D Systems) (1:50), or Sib-IR700 conjugate (10 μg/mL) for 30 minutes. .. After staining, the cells were fixed with 4% paraformaldehyde (PFA) for 30 minutes and analyzed using a FACSLyric flow cytometer (BD Biosciences Inc.), with the PE and APC-Cy7 channels.

    Staining:

    Article Title: Anakinra-Loaded Sphingomyelin Nanosystems Modulate In Vitro IL-1-Dependent Pro-Tumor Inflammation in Pancreatic Cancer
    Article Snippet: .. CAFs were characterized by staining with anti-FAP antibody (APC-conjugated mouse anti-human FAP antibody, R&D Systems, cat. FAB3715A) and anti-EpCAM antibody (PE-conjugated mouse anti-human EpCAM/CD326 antibody, BD Biosciences, San Jose, CA, USA, cat. 347198), following the manufacturer’s instructions, and data were acquired as above. .. Internalization of nanosystems in L3.6pl cells was evaluated by confocal laser microscopy (Leica Microscope SP8 ® , Wetzlar, Germany, EU).

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: Cy 5.5 fluorescence intensity in dissected organs (heart, lung, spleen, kidneys, and liver) was detected using an IVIS® Spectrum in vivo imaging system (PerkinElmer, Waltham, MA, USA). .. For histological analysis, cardiac tissues were cryosectioned 24 h post-injection and subjected to immunofluorescence staining with anti-FAP antibody (AF3715, R&D Systems, Minneapolis, MN, USA) and 4′,6-diamidino-2-phenylindole (DAPI; 62248, Thermo Fisher Scientific). .. Stained slides were visualized using a confocal microscope (Zeiss LSM 710, Carl Zeiss).

    Article Title: Anakinra-Loaded Sphingomyelin Nanosystems Modulate In Vitro IL-1-Dependent Pro-Tumor Inflammation in Pancreatic Cancer.
    Article Snippet: .. CAFs were characterized by staining with anti-FAP antibody (APC-conjugated mouse anti-human FAP antibody, R&D Systems, cat. FAB3715A) and anti-EpCAM antibody (PE-conjugated mouse anti-human EpCAM/CD326 antibody, BD Biosciences, San Jose, CA, USA, cat. 347198), following the manufacturer’s instructions, and data were acquired as above. .. Internalization of nanosystems in L3.6pl cells was evaluated by confocal laser microscopy (Leica Microscope SP8®, Wetzlar, Germany, EU).

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: Cy 5.5 fluorescence intensity in dissected organs (heart, lung, spleen, kidneys, and liver) was detected using an IVIS® Spectrum in vivo imaging system (PerkinElmer, Waltham, MA, USA). .. For histological analysis, cardiac tissues were cryosectioned 24 h post-injection and subjected to immunofluorescence staining with anti-FAP antibody (AF3715, R&D Systems, Minneapolis, MN, USA) and 4′,6-diamidino-2-phenylindole (DAPI; 62248, Thermo Fisher Scientific). .. Stained slides were visualized using a confocal microscope (Zeiss LSM 710, Carl Zeiss). https://doi.org/10.2147/IJN.S497428 International Journal of Nanomedicine 2025:20 2578 Powered by TCPDF (www.tcpdf.org) Evaluation of Toxicity in vitro and in vivo Cell viability and lactate dehydrogenase (LDH) release were evaluated using the Quint-MAXTM WST-8 Cell Viability Assay Kit (QM2500, Biomax, Seoul, Korea) and Quint-LDHTM PLUS Cytotoxicity Assay Kit (BCT-LDHP1000, Biomax) following the manufacturer’s instructions.

    Immunofluorescence:

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: Cy 5.5 fluorescence intensity in dissected organs (heart, lung, spleen, kidneys, and liver) was detected using an IVIS® Spectrum in vivo imaging system (PerkinElmer, Waltham, MA, USA). .. For histological analysis, cardiac tissues were cryosectioned 24 h post-injection and subjected to immunofluorescence staining with anti-FAP antibody (AF3715, R&D Systems, Minneapolis, MN, USA) and 4′,6-diamidino-2-phenylindole (DAPI; 62248, Thermo Fisher Scientific). .. Stained slides were visualized using a confocal microscope (Zeiss LSM 710, Carl Zeiss).

    Article Title: Injured Cardiac Tissue-Targeted Delivery of TGFβ1 siRNA by FAP Aptamer-Functionalized Extracellular Vesicles Promotes Cardiac Repair
    Article Snippet: Cy 5.5 fluorescence intensity in dissected organs (heart, lung, spleen, kidneys, and liver) was detected using an IVIS® Spectrum in vivo imaging system (PerkinElmer, Waltham, MA, USA). .. For histological analysis, cardiac tissues were cryosectioned 24 h post-injection and subjected to immunofluorescence staining with anti-FAP antibody (AF3715, R&D Systems, Minneapolis, MN, USA) and 4′,6-diamidino-2-phenylindole (DAPI; 62248, Thermo Fisher Scientific). .. Stained slides were visualized using a confocal microscope (Zeiss LSM 710, Carl Zeiss). https://doi.org/10.2147/IJN.S497428 International Journal of Nanomedicine 2025:20 2578 Powered by TCPDF (www.tcpdf.org) Evaluation of Toxicity in vitro and in vivo Cell viability and lactate dehydrogenase (LDH) release were evaluated using the Quint-MAXTM WST-8 Cell Viability Assay Kit (QM2500, Biomax, Seoul, Korea) and Quint-LDHTM PLUS Cytotoxicity Assay Kit (BCT-LDHP1000, Biomax) following the manufacturer’s instructions.



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